產(chǎn)品詳情
簡單介紹：
重組人白細(xì)胞介素-17蛋白與其它公司提供的重組蛋白不同，rHuIL-17蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細(xì)胞介素-17蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人白細(xì)胞介素-17蛋白
| Synonyms | Cytotoxic T-lymphocyte-associated antigen 8, CLTA-8 , IL-17A |
| Species | Human |
| Accession | Q16552 |
| GeneID | 3605 |
| Source | 重組人白細(xì)胞介素-17蛋白Escherichia coli. |
| Molecular Weight | Approximately 31.0 kDa, a disulfide-linked homodimer of two 132 amino acid polypeptide chains. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VA |
| Purity | 重組人白細(xì)胞介素-17蛋白> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 7.5 ng/ml, corresponding to a specific activity of > 1.3 × 105 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | 重組人白細(xì)胞介素-17蛋白Less than 1 EU/μg of rHuIL-17 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人白細(xì)胞介素-17蛋白 | |
| SDS-PAGE |
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| Reference |
1. Mungall AJ, Palmer SA, Sims SK, et al. 2003. Nature, 425: 805-11. 2. Kolls JKandLinden A. 2004. Immunity, 21: 467-76. 3. Fossiez F, Djossou O, Chomarat P, et al. 1996. J Exp Med, 183: 2593-603. 4. Yao Z, Painter SL, Fanslow WC, et al. 1995. J Immunol, 155: 5483-6. |
| Background | Human Interleukin-17A (IL-17A) is encoded by the IL17A gene located on the chromosome 6 and belongs to the IL-17 family that contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. They have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, but no sequence similarity to any other known cytokines. Interleukin 17 is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, IL-17 exhibits 63 % amino acid identity with mouse IL-17. High levels of human IL-17 were induced from primary peripheral blood CD4+ T cells upon stimulation and they can induce stromal cells to produce proinflammatory and hematopoietic cytokines. |
