產品詳情
簡單介紹：
核因子κ-B的重組人可溶性受體激活劑蛋白與其它公司提供的重組蛋白不同，rHusRANK Receptor/TNFRSF11A蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.核因子κ-B的重組人可溶性受體激活劑蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
核因子κ-B的重組人可溶性受體激活劑蛋白
| Species | Human |
| Accession | Q9Y6Q6 |
| GeneID | 8792 |
| Source | Escherichia coli. |
| Molecular Weight | 核因子κ-B的重組人可溶性受體激活劑蛋白Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
| Quantity | 20μg/100μg/1000μg |
| AA Sequence | QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 核因子κ-B的重組人可溶性受體激活劑蛋白Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 核因子κ-B的重組人可溶性受體激活劑蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl. |
| Endotoxin | Less than 0.1 EU/μg of rHusRANK Receptor as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 核因子κ-B的重組人可溶性受體激活劑蛋白 | |
| SDS-PAGE |
|
