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產品名稱：重組人成纖維細胞生長因子4蛋白
產品型號：rHuFGF-4
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人成纖維細胞生長因子4蛋白與其它公司提供的重組蛋白不同，rHuFGF-4蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人成纖維細胞生長因子4蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人成纖維細胞生長因子4蛋白

Synonyms HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3

Species Human

Accession P08620

GeneID 2249

Source Escherichia coli.

Molecular Weight 重組人成纖維細胞生長因子4蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.

Quantity 5μg/25μg/1000μg

AA Sequence GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

Purity 重組人成纖維細胞生長因子4蛋白> 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.

Endotoxin 重組人成纖維細胞生長因子4蛋白Less than 1 EU/μg of rHuFGF-4 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人成纖維細胞生長因子4蛋白

SDS-PAGE

Reference 1. Galland F, Stefanova M, Lafage M, et al. 1992. Cytogenet Cell Genet. 60:114-6.
2. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.
3. Boulet AM, Capecchi MR. 2012. Dev Biol. 371:235-45.

Background FGF-4, also named FGF-K and K-FGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF-4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF-4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family.

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Synonyms	HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3
Species	Human
Accession	P08620
GeneID	2249
Source	Escherichia coli.
Molecular Weight	重組人成纖維細胞生長因子4蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
Quantity	5μg/25μg/1000μg
AA Sequence	GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Purity	重組人成纖維細胞生長因子4蛋白> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.
Endotoxin	重組人成纖維細胞生長因子4蛋白Less than 1 EU/μg of rHuFGF-4 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人成纖維細胞生長因子4蛋白
SDS-PAGE
Reference	1. Galland F, Stefanova M, Lafage M, et al. 1992. Cytogenet Cell Genet. 60:114-6. 2. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 3. Boulet AM, Capecchi MR. 2012. Dev Biol. 371:235-45.
Background	FGF-4, also named FGF-K and K-FGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF-4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF-4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family.

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產品名稱：重組人成纖維細胞生長因子4蛋白