產(chǎn)品詳情
簡單介紹：
重組大鼠白細(xì)胞介素-22蛋白與其它公司提供的重組蛋白不同，rRtIL-22蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠白細(xì)胞介素-22蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠白細(xì)胞介素-22蛋白
| Synonyms | Cytokine Zcyto 18, TIF |
| Species | Rat |
| Accession | Q198B4 |
| Unigene | Rn.195879. |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠白細(xì)胞介素-22蛋白Approximately16.6 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | LPINSQCKLE AANFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEELFRGV KAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSIHLSPCH ISGDDQNIQK NVRQLKETVQ KLGESGEIKA IGELDLLFMS LRNACV |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠白細(xì)胞介素-22蛋白Fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rRtIL-22 as determined by LAL method. |
| Reconstitution | 重組大鼠白細(xì)胞介素-22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠白細(xì)胞介素-22蛋白 | |
| SDS-PAGE |
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| Reference |
1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9. 2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668. 3. Honda K. 2012. Immunity, 37: 952-4. 4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77. |
| Background | IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant rat Interleukin-22 contains 146 amino acids and it has approximately 79 % and 92 % amino acid sequence identity with human and murine IL-22. |
