產品詳情
簡單介紹：
重組大鼠肝素結合性EGF樣生長因子蛋白與其它公司提供的重組蛋白不同，rRtHB-EGF蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠肝素結合性EGF樣生長因子蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠肝素結合性EGF樣生長因子蛋白
| Synonyms | HBEGF, DT-R |
| Species | Rat |
| Accession | Q06175 |
| GeneID | 25433 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠肝素結合性EGF樣生長因子蛋白Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. |
| Quantity | 10μg/50μg/1000μg |
| AA Sequence | DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠肝素結合性EGF樣生長因子蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose. |
| Endotoxin | 重組大鼠肝素結合性EGF樣生長因子蛋白Less than 1 EU/μg of rRtHB-EGF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠肝素結合性EGF樣生長因子蛋白 | |
| Reference |
1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73. 2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9. |
| Background | Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Rat HB-EGF shares about 76 % a.a. sequence identity with human HB-EGF. |
