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產品名稱：重組人胰島素樣生長因子- 1蛋白
產品型號：rHuIGF-1
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人胰島素樣生長因子- 1蛋白與其它公司提供的重組蛋白不同，rHuIGF-1蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人胰島素樣生長因子- 1蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人胰島素樣生長因子- 1蛋白

Synonyms Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF

Species Human

Accession 重組人胰島素樣生長因子- 1蛋白P05019

GeneID 3479

Source Escherichia coli.

Molecular Weight Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

Quantity 100μg/500μg/1000μg

AA Sequence 重組人胰島素樣生長因子- 1蛋白GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity Assay #1: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.
Assay #2: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

Physical Appearance 重組人胰島素樣生長因子- 1蛋白Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Endotoxin Less than 0.01 EU/μg of rHuIGF-1 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable for 3 years at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to two weeks at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人胰島素樣生長因子- 1蛋白

SDS-PAGE

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Synonyms	Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF
Species	Human
Accession	重組人胰島素樣生長因子- 1蛋白P05019
GeneID	3479
Source	Escherichia coli.
Molecular Weight	Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
Quantity	100μg/500μg/1000μg
AA Sequence	重組人胰島素樣生長因子- 1蛋白GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	Assay #1: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg. Assay #2: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.
Physical Appearance	重組人胰島素樣生長因子- 1蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
Endotoxin	Less than 0.01 EU/μg of rHuIGF-1 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable for 3 years at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to two weeks at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人胰島素樣生長因子- 1蛋白
SDS-PAGE

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產品名稱：重組人胰島素樣生長因子- 1蛋白