產(chǎn)品詳情
簡單介紹：
重組體人Interleukin-36γ蛋白與其它公司提供的重組蛋白不同，rHuIL-36γ蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組體人Interleukin-36γ蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組體人Interleukin-36γ蛋白
| Synonyms | IL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1 |
| Species | Human |
| Accession | Q9NZH8 |
| GeneID | 56300 |
| Source | 重組體人Interleukin-36γ蛋白Escherichia coli. |
| Molecular Weight | Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 169 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MRGTPGDADG GGRAVYQSMC KPITGTINDL NQQVWTLQGQ NLVAVPRSDS VTPVTVAVIT CKYPEALEQG RGDPIYLGIQ NPEMCLYCEK VGEQPTLQLK EQKIMDLYGQ PEPVKPFLFY RAKTGRTSTL ESVAFPDWFI ASSKRDQPII LTSELGKSYN TAFELNIND |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組體人Interleukin-36γ蛋白Fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36γ at 1 μg/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 μg/mL. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuIL-36γ, 169a.a. as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組體人Interleukin-36γ蛋白 | |
| Reference |
1. Nicklin MJ, Barton JL, Nguyen M, et al. 2002. Genomics. 79:718-25. 2. Dinarello C, Arend W, Sims J, et al. 2010. Nat Immunol. 11:973. 3. Debets R, Timans JC, Homey B, et al. 2001. J Immunol. 167:1440-6. 4. Busfield SJ, Comrack CA, Yu G, et al. 2000. Genomics. 66:213-6. 5. Clark HF, Gurney AL, Abaya E, et al. 2003. Genome Res. 13:2265-70. |
| Background | 重組體人Interleukin-36γ蛋白Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36γ is secreted when transfected into 293-T cells and it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). Furthermore, IL-36γ also can function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Recombinant human IL-36γ is synthesized as a 19 kDa, 169 amino acid (a.a.) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site. Human to mouse, IL-36γ shares 53 % a.a. identity. Within the family, IL-36γ shares about 25 % ~ 55 % a.a. sequence identity with IL-1RA, IL-1β, IL-36RA, IL-36α, IL-37, IL-36β and IL-1F10. |
