產品詳情
簡單介紹：
重組人I-309 / CCL1蛋白與其它公司提供的重組蛋白不同，rHuI-309/CCL1蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人I-309 / CCL1蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人I-309 / CCL1蛋白
| Synonyms | Small-inducible cytokine A1, T lymphocyte-secreted protein I-309 |
| Species | Human |
| Accession | P22362 |
| GeneID | 6346 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人I-309 / CCL1蛋白Approximately 8.6 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人I-309 / CCL1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuI-309/CCL1 as determined by LAL method. |
| Reconstitution | 重組人I-309 / CCL1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人I-309 / CCL1蛋白 | |
| SDS-PAGE |
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| Reference |
1. Miller MD, Krangel MS. 1992. Proc Natl Acad Sci U S A. 89:2950-4. 2. Roos RS, Loetscher M, Legler DF, et al. 1997. J Biol Chem. 272:17251-4. 3. Miller MD, Wilson SD, Dorf ME, et al. 1990. J Immunol. 145:2737-44. |
| Background | Chemokine (C-C motif) ligand 1 (CCL1) belongs to a family inflammatory cytokines and also known as chemokines. It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5 kDa. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42 % amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. |
