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  • 產品名稱:重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白

  • 產品型號:rHuENA-78, 8-78 a.a./CXCL5
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白與其它公司提供的重組蛋白不同,rHuENA-78, 8-78 a.a./CXCL5蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白

Synonyms CXCL5, Small-inducible cytokine B5
Species Human
Accession P42830
GeneID 6374
Source 重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白Escherichia coli.
Molecular Weight Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10.0-100.0 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin Less than 1 EU/μg of rHuENA-78, 8 - 78 a.a./CXCL5 as determined by LAL method.
Reconstitution 重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白
Reference 1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7.
4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.
Background CXCL5 is a member of the CXC chemokine family and also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. In vitro, ENA-78 (8-78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. They are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Recombinant human CXCL5 (8-78 a.a.) contains 71 amino acids which is a single non-glycosylated polypeptide chain. Human CXCL5 shares 57 % amino acid sequence identity with mouse and rat CXCL5.

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