產品詳情
簡單介紹：
重組人嗜中性粒細胞活化蛋白-2 / CXCL7蛋白與其它公司提供的重組蛋白不同，rHuNAP-2/CXCL7蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人嗜中性粒細胞活化蛋白-2 / CXCL7蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人嗜中性粒細胞活化蛋白-2 / CXCL7蛋白
| Synonyms | CXCL7, LDGF, MDGF, Small-inducible cytokine B7 |
| Species | Human |
| Accession | P02775 |
| GeneID | 5473 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人嗜中性粒細胞活化蛋白-2 / CXCL7蛋白Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 1.0-10.0 ng/ml. |
| Physical Appearance | 重組人嗜中性粒細胞活化蛋白-2 / CXCL7蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuNAP-2/CXCL7 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組人嗜中性粒細胞活化蛋白-2 / CXCL7蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人嗜中性粒細胞活化蛋白-2 / CXCL7蛋白 | |
| Reference |
1. Hristov M, Zernecke A, Bidzhekov K, et al. 2007. Circ Res. 100:590-7. 2. Majumdar S, Gonder D, Koutsis B, et al. 1991. J Biol Chem. 266:5785-9. 3. Krijgsveld J, Zaat SA, Meeldijk J, et al. 2000. J Biol Chem. 275:20374-81. 4. Piccardoni P, Evangelista V, Piccoli A, et al. 1996. Thromb Haemost. 76:780-5. |
| Background | Neutrophil activating protein-2 also named CXCL7 is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein. It belongs to the CXC chemokine family and is released in large amounts from platelets following their activation. CXCL7 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. Recombinant human CXCL7 contains 70 amino acids which is a single non-glycosylated polypeptide chain. In addition, The human CXCL7 shares 53 % and 58 % a.a. sequence identity with mouse and rat CXCL7. |
