產(chǎn)品詳情
簡單介紹：
重組人白細胞介素-17F蛋白與其它公司提供的重組蛋白不同，rHuIL-17F蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細胞介素-17F蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人白細胞介素-17F蛋白
| Synonyms | ML-1, IL-24 |
| Species | Human |
| Accession | Q96PD4 |
| GeneID | 112744 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人白細胞介素-17F蛋白Approximately 30.1 kDa, a disulfide-linked homodimer of two 134 amino acid polypeptide chains. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人白細胞介素-17F蛋白Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 × 104 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuIL-17F as determined by LAL method. |
| Reconstitution | 重組人白細胞介素-17F蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 6 mM HCl to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人白細胞介素-17F蛋白 | |
| SDS-PAGE |
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| Reference |
1. Mungall AJ, Palmer SA, Sims SK, et al. 2003. Nature 425: 805-11. 2. Kolls JK, Linden A. 2004. Immunity 21: 467-76. 3. Hymowitz SG, Filvaroff EH, Yin JP, et al. 2001. EMBO J 20: 5332-41. 4. Starnes T, Robertson MJ, Sledge G, et al. 2001. J Immunol 167: 4137-40. |
| Background | Human Interleukin-17F (IL-17F) is encoded by the IL17F gene located on the chromosome 6 and belongs to the IL-17 family which contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17F that shares 50 % homologous of crystal structure to IL-17A and is expressed by activated T cells, has the functions of stimulating production of other cytokines (such as IL-6, IL-8 and granulocyte colony-stimulating factor) and proliferation of PBMC and T-cell, regulating cartilage matrix turnover, and inhibiting angiogenesis. |
