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產品名稱：重組人白介素-31蛋白
產品型號：rHuIL-31
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人白介素-31蛋白與其它公司提供的重組蛋白不同，rHuIL-31蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白介素-31蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人白介素-31蛋白

Species Human

Accession Q6EBC2

GeneID 386653

Source Escherichia coli.

Molecular Weight Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence 重組人白介素-31蛋白SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng/mL of rHuIL-31 can effectively induce STAT3 activation.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 重組人白介素-31蛋白Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.

Endotoxin Less than 1 EU/μg of rHuIL-31 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組人白介素-31蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人白介素-31蛋白

Reference 1. Sonkoly E, Muller A, Lauerma AI, et al. 2006. J Allergy Clin Immunol, 117: 411-7.
2. Jawa RS, Chattopadhyay S, Tracy E, et al. 2008. J Interferon Cytokine Res, 28: 207-19.
3. Takaoka A, Arai I, Sugimoto M, et al. 2005. Eur J Pharmacol, 516: 180-1.
4. Ohmatsu H, Sugaya M, Suga H, et al. 2012. Acta Derm Venereol, 92: 282-3

Background Human IL-31 gene is located on Chr.12. It expresses the IL-31 protein at low levels in the type 2 helper T cells, which exits in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. This protein shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. IL-31 signals through IL-31 receptor A and oncostatin M receptor subunits and can activate STAT3 through receptors and maybe involve in skin immunity. It regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Human IL-31 shares 24 % a.a. sequence identity in the mature protein with mouse IL-31.

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Species	Human
Accession	Q6EBC2
GeneID	386653
Source	Escherichia coli.
Molecular Weight	Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	重組人白介素-31蛋白SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng/mL of rHuIL-31 can effectively induce STAT3 activation.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重組人白介素-31蛋白Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
Endotoxin	Less than 1 EU/μg of rHuIL-31 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組人白介素-31蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人白介素-31蛋白
Reference	1. Sonkoly E, Muller A, Lauerma AI, et al. 2006. J Allergy Clin Immunol, 117: 411-7. 2. Jawa RS, Chattopadhyay S, Tracy E, et al. 2008. J Interferon Cytokine Res, 28: 207-19. 3. Takaoka A, Arai I, Sugimoto M, et al. 2005. Eur J Pharmacol, 516: 180-1. 4. Ohmatsu H, Sugaya M, Suga H, et al. 2012. Acta Derm Venereol, 92: 282-3
Background	Human IL-31 gene is located on Chr.12. It expresses the IL-31 protein at low levels in the type 2 helper T cells, which exits in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. This protein shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. IL-31 signals through IL-31 receptor A and oncostatin M receptor subunits and can activate STAT3 through receptors and maybe involve in skin immunity. It regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Human IL-31 shares 24 % a.a. sequence identity in the mature protein with mouse IL-31.

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產品名稱：重組人白介素-31蛋白