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產品名稱：重組人粒細胞集落刺激因子蛋白
產品型號：rHuG-CSF
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人粒細胞集落刺激因子蛋白與其它公司提供的重組蛋白不同，rHuG-CSF蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人粒細胞集落刺激因子蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人粒細胞集落刺激因子蛋白

Synonyms CSF-3, MGI-1G, GM-CSF beta, Pluripoietin

Species Human

Accession P09919

GeneID 1440

Source Escherichia coli.

Molecular Weight 重組人粒細胞集落刺激因子蛋白Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人粒細胞集落刺激因子蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5 % trehalose, pH 4.0.

Endotoxin Less than 1 EU/μg of rHuG-CSF as determined by LAL method.

Reconstitution 重組人粒細胞集落刺激因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人粒細胞集落刺激因子蛋白

SDS-PAGE

Reference 1. Scarffe JHandKamthan A. 1990. Cancer Surv, 9: 115-30.
2. Fattorini L, Xiao Y, Li B, et al. 1994. J Med Microbiol, 40: 129-33.
3. Tsavaris N, Kosmas C, Gouveris P, et al. 2004. Med Sci Monit, 10: PI24-8.
4. Shochat E, Rom-Kedar V, Segel LA. 2007. Bull Math Biol, 69: 2299-338.
5. Cornish AL, Campbell IK, McKenzie BS, et al. 2009. Nat Rev Rheumatol, 5: 554-9.

Background Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition it may function in some adhesion or recognition events at the cell surface. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (a.a.) precursor proteins, have been isolated. Both proteins have a 30 a.a. signal peptide and have identical amino acid sequences except for a three a.a. insertion (deletion) at the 35th a.a. residue from the N-terminus of the mature protein. Human G-CSF is 73 % identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

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Synonyms	CSF-3, MGI-1G, GM-CSF beta, Pluripoietin
Species	Human
Accession	P09919
GeneID	1440
Source	Escherichia coli.
Molecular Weight	重組人粒細胞集落刺激因子蛋白Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人粒細胞集落刺激因子蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5 % trehalose, pH 4.0.
Endotoxin	Less than 1 EU/μg of rHuG-CSF as determined by LAL method.
Reconstitution	重組人粒細胞集落刺激因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人粒細胞集落刺激因子蛋白
SDS-PAGE
Reference	1. Scarffe JHandKamthan A. 1990. Cancer Surv, 9: 115-30. 2. Fattorini L, Xiao Y, Li B, et al. 1994. J Med Microbiol, 40: 129-33. 3. Tsavaris N, Kosmas C, Gouveris P, et al. 2004. Med Sci Monit, 10: PI24-8. 4. Shochat E, Rom-Kedar V, Segel LA. 2007. Bull Math Biol, 69: 2299-338. 5. Cornish AL, Campbell IK, McKenzie BS, et al. 2009. Nat Rev Rheumatol, 5: 554-9.
Background	Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition it may function in some adhesion or recognition events at the cell surface. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (a.a.) precursor proteins, have been isolated. Both proteins have a 30 a.a. signal peptide and have identical amino acid sequences except for a three a.a. insertion (deletion) at the 35th a.a. residue from the N-terminus of the mature protein. Human G-CSF is 73 % identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

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產品名稱：重組人粒細胞集落刺激因子蛋白