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產品名稱：重組恒河猴獼猴白介素-5蛋白
產品型號：rRhIL-5
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組恒河猴獼猴白介素-5蛋白與其它公司提供的重組蛋白不同，rRhIL-5蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組恒河猴獼猴白介素-5蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組恒河猴獼猴白介素-5蛋白

Synonyms Eosinophil differentiation factor, TRF

Species Rhesus Macaque

Accession P48093

GeneID 710622

Unigene Mmu.3362.

Source 重組恒河猴獼猴白介素-5蛋白Escherichia coli.

Molecular Weight Approximately 26.1 kDa, a disulfide-linked homodimer of two 115 amino acid polypeptide chains.

AA Sequence IPTEIPASAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYIG GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組恒河猴獼猴白介素-5蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.

Endotoxin 重組恒河猴獼猴白介素-5蛋白Less than 0.1 EU/μg of rRhIL-5 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組恒河猴獼猴白介素-5蛋白

Background Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. IL-5 is a 115-amino acid (in rhesus macaque, 133 in the murine) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.

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Synonyms	Eosinophil differentiation factor, TRF
Species	Rhesus Macaque
Accession	P48093
GeneID	710622
Unigene	Mmu.3362.
Source	重組恒河猴獼猴白介素-5蛋白Escherichia coli.
Molecular Weight	Approximately 26.1 kDa, a disulfide-linked homodimer of two 115 amino acid polypeptide chains.
AA Sequence	IPTEIPASAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYIG GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組恒河猴獼猴白介素-5蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.
Endotoxin	重組恒河猴獼猴白介素-5蛋白Less than 0.1 EU/μg of rRhIL-5 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組恒河猴獼猴白介素-5蛋白
Background	Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. IL-5 is a 115-amino acid (in rhesus macaque, 133 in the murine) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.

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產品名稱：重組恒河猴獼猴白介素-5蛋白