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產品名稱：重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白
產品型號：rMuGDF-7/BMP-12
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白與其它公司提供的重組蛋白不同，rMuGDF-7/BMP-12蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白

Species Murine

Accession P43029

GeneID 238057

Source Escherichia coli.

Molecular Weight 重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.

AA Sequence 重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Endotoxin Less than 0.1 EU/μg of rMuGDF-7/BMP-12 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白

Background Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

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Species	Murine
Accession	P43029
GeneID	238057
Source	Escherichia coli.
Molecular Weight	重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.
AA Sequence	重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Endotoxin	Less than 0.1 EU/μg of rMuGDF-7/BMP-12 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白
Background	Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

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產品名稱：重組小鼠生長分化因子7 /骨形態發生蛋白-12蛋白