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產品名稱：重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白
產品型號：rMuGDF-5/BMP-14
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白與其它公司提供的重組蛋白不同，rMuGDF-5/BMP-14蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白

Species Murine

Accession P43027

GeneID 14563

Source 重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白Escherichia coli.

Molecular Weight Approximately 27.2 kDa, a disulfide-linked homodimeric protein containing two 120 amino acids.

AA Sequence APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

Purity 重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白> 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Endotoxin Less than 0.1 EU/μg of rMuGDF-5/BMP-14 as determined by LAL method.

Reconstitution 重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白

Background Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

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Species	Murine
Accession	P43027
GeneID	14563
Source	重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白Escherichia coli.
Molecular Weight	Approximately 27.2 kDa, a disulfide-linked homodimeric protein containing two 120 amino acids.
AA Sequence	APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
Purity	重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Endotoxin	Less than 0.1 EU/μg of rMuGDF-5/BMP-14 as determined by LAL method.
Reconstitution	重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白
Background	Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

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產品名稱：重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白