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產品名稱：重組大鼠膠質細胞來源的神經營養因子蛋白
產品型號：rRtGDNF
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠膠質細胞來源的神經營養因子蛋白與其它公司提供的重組蛋白不同，rRtGDNF蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠膠質細胞來源的神經營養因子蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠膠質細胞來源的神經營養因子蛋白

Synonyms ATF

Species Rat

Accession Q07731

GeneID 25453

Source Escherichia coli.

Molecular Weight 重組大鼠膠質細胞來源的神經營養因子蛋白Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

Quantity 2μg/10μg/1000μg

AA Sequence SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI

Purity 重組大鼠膠質細胞來源的神經營養因子蛋白> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.

Endotoxin 重組大鼠膠質細胞來源的神經營養因子蛋白Less than 0.1 EU/μg of rRtGDNF as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠膠質細胞來源的神經營養因子蛋白

Reference 1. Lin LF, Doherty DH, Lile JD, et al. 1993. Science. 260:1130-2.
2. Russell FD, Koishi K, Jiang Y, et al. 2000. Neuroscience. 97:575-80.
3. Jing S, Yu Y, Fang M, et al. 1997. J Biol Chem. 272:33111-7.
4. Cik M, Masure S, Lesage AS, et al. 2000. J Biol Chem. 275:27505-12.

Background Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. GDNF Recombinant rat GDNF (monomer) contains 135 amino acids residues, which is a disulfide-linked homodimer and it shares 99 % and 93 % a.a. sequence identity with mouse and human GDNF.

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Synonyms	ATF
Species	Rat
Accession	Q07731
GeneID	25453
Source	Escherichia coli.
Molecular Weight	重組大鼠膠質細胞來源的神經營養因子蛋白Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Quantity	2μg/10μg/1000μg
AA Sequence	SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI
Purity	重組大鼠膠質細胞來源的神經營養因子蛋白> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.
Endotoxin	重組大鼠膠質細胞來源的神經營養因子蛋白Less than 0.1 EU/μg of rRtGDNF as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠膠質細胞來源的神經營養因子蛋白
Reference	1. Lin LF, Doherty DH, Lile JD, et al. 1993. Science. 260:1130-2. 2. Russell FD, Koishi K, Jiang Y, et al. 2000. Neuroscience. 97:575-80. 3. Jing S, Yu Y, Fang M, et al. 1997. J Biol Chem. 272:33111-7. 4. Cik M, Masure S, Lesage AS, et al. 2000. J Biol Chem. 275:27505-12.
Background	Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. GDNF Recombinant rat GDNF (monomer) contains 135 amino acids residues, which is a disulfide-linked homodimer and it shares 99 % and 93 % a.a. sequence identity with mouse and human GDNF.

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產品名稱：重組大鼠膠質細胞來源的神經營養因子蛋白