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產(chǎn)品詳情
  • 產(chǎn)品名稱:重組大鼠巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白

  • 產(chǎn)品型號:rRtMIP-1α/CCL3
  • 產(chǎn)品廠商:KALANG
  • 產(chǎn)品文檔:
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簡單介紹:
重組大鼠巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白與其它公司提供的重組蛋白不同,rRtMIP-1α/CCL3蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白(全長序列)產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組大鼠巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白

Species Rat
Accession P50229
GeneID 25542
Source Escherichia coli.
Molecular Weight 重組大鼠巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA
Purity > 98 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組大鼠巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human blood monocytes is in a concentration range of 10-100 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Endotoxin 重組大鼠巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白Less than 0.1 EU/μg of rRtMIP-1α/CCL3 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白

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